EZ-DNA Genomic DNA Isolation Kit

EZ-DNA is a non-organic and ready to use reagent for the isolation of genomic DNA from samples of human, animal, plant, yeast, bacterial and viral origin. EZ-DNA is based on disruption of cells in guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from a cell lysate with ethanol. Following an ethanol wash, DNA is solubilized in water or 8 mM NaOH. There is no phenol in EZ-DNA. The protocols fast and permits isolation of genomic DNA from a large number of samples of small or large volumes. The procedure can be completed in 10-30 minutes with DNA recovery of 70-100%. The isolated DNA can be used, without additional purification, for southern analysis, dot blot hybridization, molecular cloning, RFLP, PCR and other molecular biology and biotechnology applications.

Kits Reagents

· 50ml solution containing Guanidinium Isothiocynate

Storage

· EZ-DNA should be stored at room temperature. Storing at lower temperatures will cause the Guanidine Isothiocyanate to come out of the solution. If the reagent is warmed, the Guanidine Isothiocyanate should resolubilized instantly.

Assessing yield of genomic DNA

The yield of genomic DNA will vary depending on the tissue or cells from which it is obtained.

Tissue/Sample type	Amount of starting material	Yield of total RNA
Liver	1 mg	3-4µg
Kidney	1 mg	3-4µg
Skeletal muscle	1 mg	2-3µg
Brain	1 mg	2-3µg
Placenta	1 mg	2-3µg
Human cells	10 cells	5-7µg
Rat cells	10 cells	5-7µg
Mouse cells	10 cells	5-7µg
Lung	1 mg	3-5µg
Heart	1 mg	2-3µg
Plant leaf	1 gr	20-200µg
Whole blood	1 ml	20-40µg
Sf9 cells	10 cells	170-180
E.coli cells	10 cells	30-40µg
Mouse tail	1 mg	0.4-3µg

For complete Product Protocol click here