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EZ-First Strand cDNA Synthesis Kit
Premixed solutions for the synthesis of single-stranded cDNA from RNA for use as a PCR template
Using the EZ-First Strand cDNA
Synthesis Kit, RNA is reverse-transcribed into single-stranded cDNA. The reverse
transcriptase (RT) enzyme synthesizes the new cDNA strand at a site determined
by the type of primer used: oligo (dT) primer, random primer or a
sequence-specific primer.
The first strand cDNA can then be used as a template for PCR. The reaction mix solution supplied contains buffer, MMLV reverse transcriptase (point mutant lacking RNase H activity), and RNase inhibitor sufficient for 50 reactions.
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