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Life Technologies India -
Services | |
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Cell
Culture
Maintenance
Expansion
Transfection
Expression
Separation
Banking Virology
Propagation of Human & Animal Viruses
Isolation of Viral Protein, RNA and DNA
Plaque Assay and TCID5O Services Virus
Expression Systems
Titering Concentrated Virus for:
o
Confirmation of viral
stocks viability
o
Determination of proper
transduction conditions
o
Determination of
maximum number of target cells
o
Determination of virus
permissivity
Adenovirus and adeno-associated virus (AAV)
services
o
Construction of high
titer stocks of recombinant viruses
o
Construction
recombinant AAV General
Molecular Mutagenesis
Transformation
Cloning
Sequencing
Library Construction
Library Screening RNase
Protection Assay FISH
Analysis
Chromosome Localization
Transgene Analysis Gene
Mapping In
Situ Hybridization PCR
Endpoint PCR
o
Adventitious Virus
Testing
o
Murine PCR (Replacing
MAP Testing)
o
Transgenic
PCR
QPCR
o
Full-Service
Taqmanฎ
o
Host Cell DNA
Assays
o
Integration
o
Biodistribution Transfection
Vector Construction
Transfection
Electroporation
Lipofection Tr~nsfection
Insert Confirmation
Selection and Enrichment
Clonal Selection Gene
Expression
Generating Expression Construct
Generating Recombinant Virus
Synthesize the Desired Gene
Sub-cloning the Gene into an Expression
Vector
Establishing High-expression Stable Cell
Lines
Small-scale Expression and Purification
Optimization of Expression Conditions Large-scale Expression and Purification Protein Purification
Protein A and IgG-Fc Fusion Protein
Customized Purification
o
Affinity
column
o
Gel
filtration column
o
Ion
exchange column
o
Hydrophobic
column
o
Antibody
purification Protein
Analysis
Sample Preparation
Protein Determination TCA
and Ethanol Precipitation
Micro-dialysis
Nuclease Treatment
Enzymatic Deglycosylation Gel
Electrophoresis
Glycoprotein Analysis
Epitope Mapping Plasmid
and Phage Large
Scale Plasmid and Phage DNA Production
Insert Isolation & Purification DNA
Immunization ELISA
Colorimetric
Fluorescent
Chemiluminescent
Competitive Enzyme Immunoassays (small
molecules)
Immunometric / Sandwich Assays (large
Molecules
Particle Based
Flow Cytometry
Immunophenotyping
Immunopharmacology
Polyclonal Antibody Production
Monoclonal Antibody Production
Humanized Mouse Antibody
Antibody Purification SNP
and SIR Genotyping
Transgenic Identification
Karyotyping Cell
Line Characterization Sex
Determination DNA
Fingerprinting AFLP
(Amplified Fragment Length Polymorphism) RFLP
(Restriction Fragment Length Polymorphism) SSR
(Simple Sequence Repeat) EST
(Expressed Sequence Tag) SNP
(Single Nucleotide Polymorphism) Genetic
Stability
Master Cell Banks (MCB)
Master Working Cell Banks (MWCB) End
of Production Cells (EPC) Host
Cell DNA Contamination Assay Metabolism
Assays
Drug-Drug Interaction Studies
Cytochrome P450 Induction
Cytochrome P450 Inhibition In
Vitro Absorption and
Metabolism Models Flux rate determination and permeability constants |
Cell Based Assays
Hemolytic Potential in Human Blood: Using human
blood
Hemolytic Potential in Rat, Dog, and Human
Blood
Optional Plasma and Serum Compatibility: Compatibility is
determined by adding the test article to plasma and serum individually and
then assessing the occurrence or non-occurrence of precipitation or
coagulation of plasma or serum protein lsoenzyme
Analysis
Glucose-6-Phosphate Dehydrogenase (G6PD)
Lactate Dehydrogenase (LD)
Malate Dehydrogenase (MD)
Nucleoside Phosphorylase (NP)
Aspartate Aminotransferase (AST)
Peptidase B (PepB)
G-Protein Coupled Receptors (GPCR) cAMP
Phosphodiesterase Assay
Protein Kinase Assay (PK) Mutagenesis COMET
Assay! DNA Damage Assay
Unscheduled DNA Synthesis (UDS) DNA
Damage
Sister Chromatid Exchange (SCE)
Bacterial Gene Mutations
Mammalian Gene Mutations
Chromosome Aberrations
Micronucleus (IVM) Test TK
in Mouse Lymphoma Cells
(L5178Y cells) 1K in
Human Lymphoblastoid TK6 Cells o
HGPRT o
CHO o
V79
cells o
L5178Y
cells Toxicity
Studies
Angiogenesis
Apoptosis
Proliferation Na!K
AlPase Activity Ca
AlPase Activity
Histone acetylase! Deacetylase
Tissue Marker Identity Safety/Toxicology Acute
Single Dose Acute
Repeat Dose
Inhalation (Microspray)
Dermal Studies Acute
Irritation Studies
Sub-Chronic Toxicity
Chronic Toxicity
ADME/PK
Immunotoxicology Immunogenicity
/ Antigenicity
Development of Anti-drug Antibodies (ADA)
Vaccine Efficacy Biodistribution
PK
QPCR
Radio-labeling
ADME Tumor
Studies
Genetic Toxicity Drug Efficacy Studies In
Vivo Mutagenesis
Unscheduled DNA Synthesis (UDS) in rat and!or mouse
liver COMET
Assay! DNA Damage Assay in rodents
Sister Chromatid Exchange SCE)
Micronuclei Formation Cell
Transformation Tumor
Response
Necropsies
Serology
Parasitology
Bacteriology
Mycology
Mycoplasma
Murine MAP-PCR
Diagnostic PCR Water
Microbiology Hematology
Complete Blood Count (CBC) Serum
Chemistry
Coagulation Blood
Glucose
Capillary Tubes Blood
Chemistry
Electrolyte Balance
Carbohydrate Metabolism Liver
function
Kidney Function
Cardiac Function Lipid
Panel Bone
and Joint
Anemia Other
Markers Endocrinology
Diabetes
Thyroid Disease
Adrenal Dysfunction
Fertility and Reproduction Bone
! Mineral Metabolism
Auto-immune Diseases Other
Bio Markers Urine
and Fecal Analyses Urine
Analyses Fecal
Analyses:
Parasitological
Microbiology Histology
Frozen Sections
Specimen Fixation
Paraffin Sections Resin
Sections
Sample Preparation
Fixative Reagents Experimental
Pathology
Sentinel Screening
Toxicology Assessment of Organs
Morphometric Evaluation Immunohistochemistry
Avidin-Biotin Methods
Alkaline Phosphatase
Antigen Retrieval
Medicated Retrieval
Proteolytic Enzyme Digestion
Sample Preparation |