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| Tech. / Comm. Queries |
Cell Proliferation Kit (XTT)
The use of XTT greatly simplifies
the procedure of measuring proliferation, and is, therefore, an excellent
solution for the quantitating of cells and their viability without using
radioactive radioactive isotopes. This kit was developed to assay cell
proliferation in reaction to different growth factors, cytokines and nutrient
components. In addition, it is suitable for assaying cytotoxicity of materials
such as TNF or other growth inhibitors. XTT can be used as a non-radioactive
substitute for cytotoxic tests based on the release of Cromium51 from cells with
no less sensitivity.
The advantages of using this kit can be summarized as follows:
· Easy-to-use there is no need
for additional reagents and/or cell washing procedures
· Speed-
multiwell plants and an ELISA reader can be used for reading
· Sensitivity- can be assayed even in low cell
concentrations
· Accuracy- dye absorbance is proportional to the number
of cells in each well
· Safety- there is no need for radioactive
isotopes
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Speed,
Sensitivity, Accuracy, Safe & Easy-to-use