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Introduction:
Acetosyringone is a naturally occurring compound secreted from wounded dicot plant tissues and is a chemotractant for Agrobacterium sp.. It is generally used at 5uM to 200uM to enhance transformation in monocots, 3',5'-Dimethyoxy-4'-hydroxyacetophenone Acetosyringone should NOT be autoclaved.
A104 Acetosyringone
| Synonyms: | 4-Hydroxy-3’,5’-dimethoxyacetophenone |
| CAS: | 2478-38-8 |
| Formula: | C10H12O4 |
| Mol. Weight: | 196.20 |
Properties
| Form: | Powder |
| Appearance: | Off-white to Tan |
| Application: | Molecular Biology |
| Solubility: | DMSO |
| Typical Working Concentration: | 50 µM to 200 µM |
| Storage Temp: | Room Temperature |
| Storage Temp of Stock Solution: | -20 to 0ºC |
| Other Notes: | Plant Tissue Culture Tested. Heat sensitive, do not autoclave. |
Application Notes
Acetosyringone is a naturally occurring compound secreted from wounded plant tissues. It is used to increase the rate of transformation when used at 50 µM to 200 µM. (Sheikholeslam & Weeks, 1987).
References
João KHL and TA Brown (1993) Enhanced transformation of tomato co-cultivated with Agrobacterium tumefaciens C58C1Rifr::pGSFR1161 in the presence of acetosyringone. Plant Cell Reports, 12:7-8, pp. 422-425.
Mathews H, Bharathan N, Litz RE, Narayanan KR, Rao PS and Bhatia CR (1990) The promotion of Agrobacterium mediated transformation in Atropa belladonna L. by acetosyringone. Journal of Plant Physiology, 136 (4). pp. 404-409. ISSN 0176-1617.
Sheikholeslam, SN & DP Weeks. 1987. Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Molecular Biology 8:291-298.
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |