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Introduction:
Acetosyringone is a naturally occurring compound secreted from wounded dicot plant tissues and is a chemotractant for Agrobacterium sp. It is generally used at 5 uM to 200 uM to enhance transformation in monocots.Acetosyringone should NOT be autoclaved. This is a sterile solution of Acetosyringone in DMSO.
Synonym: 3',5'-Dimethyoxy-4'-hydroxyacetophenone
A1104 Acetosyringone 100 mM Solution
| Synonyms: | 4-Hydroxy-3’,5’-dimethoxyacetophenone |
| CAS: | 2478-38-8 |
| Formula: | C10H12O4 |
| Mol. Weight: | 196.20 |
Properties
| Form: | Liquid |
| Appearance: | Off-white to Tan |
| Application: | Molecular Biology |
| Solubility: | Miscible in Water |
| Typical Working Concentration: | 50 µM to 200 µM |
| Storage Temp: | -20 °C |
| Storage Temp of Stock Solution: | N/A |
| Other Notes: | Plant Tissue Culture Tested |
Application Notes
This material is sensitive to temperature and should be added after autoclaved media has cooled slightly. Acetosyringone is a naturally occurring compound secreted from wounded plant tissues. It is used to increase the rate of transformation when used at 50 µM to 200 µM. (Sheikholeslam & Weeks, 1987).
References
Sheikholeslam, SN & DP Weeks. 1987. Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Molecular Biology 8:291- 298.
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |