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Introduction:
Amphotericin B is a polyene macrolide antifungal derived from Streptomyces nodosus. Its mechanism is to bind to ergosterols, lipids essential for survival. Nutrient loss due to membrane
Amphotericin B is effective against many fungi, yeasts and molds.
A119 Amphotericin B
| Synonyms: | N/A |
| CAS: | 1397-89-3 |
| Formula: | C47H73NO17 |
| Mol. Weight: | 924.09 |
Properties
| Form: | Powder |
| Appearance: | Yellow Powder |
| Application: | Plant Tissue Culture Antimycotic |
| Solubility: | Soluble in DMSO |
| Storage Temp: | 2-8°C |
| Other Notes: | Protect from Light. |
Application Notes
Amphotericin B is a polyene antimycotic derived from Streptomyces nodosus. Its mode of action is to disrupt the osmosis of the cell membrane by binding to fungal and bacterial ergosterols causing intercellular potassium and magnesium to leak out of target cells.2-4 Amphotericin B is effective against many fungi, yeasts and molds, e.g., Absidia spp., Aspergillus spp., Basidiobolus spp., Candida spp., etc.2
Minimum inhibitory concentration (MIC) of amphotericin B has been reported for many fungi, yeasts and molds. MIC for Candida spp. range from 0.1 to 2 mg/L, Aspergillus niger is 1 mg/L, Fusarium sp. is 1 to greater than 4 mg/L, Bipolaris sp. is 0.5 to 1 mg/L, etc.4
Please Note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.
References
1.Merck 13, 590
2.Martindale: The Complete Drug Reference, 35th ed., Paul S. Blake, Ed. (Royal Pharmaceutical Society, 2007), p. 471.
3.Brajtburg, Janina, William G. Powderly, George S. Kobayashi, and Gerald Medoff. 1990. Amphotericin B: current understanding of mechanisms of action. Antimicrobial Agents and Chemotheraphy. Vol. 34(2). Pp. 183-188.
4.Ellis, David. 2002. Amphotericin B: spectrum and resistance. Journal of Antimicrobial Chemotherapy. Vol 49. Suppl S1, Pp. 7-10.
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |