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Introduction:
Do you have to add components to MS-media for Arabidopsis seed germination? New to PhytoTech, product A1375 is a complete formulation for Arabidopsis, containing all the components needed for seed germination.
This formulation contains ½ MS-basal media with 0.5 g/L MES and 10 g/L sucrose. If you prefer 1x MS for Arabidopsis germination, we would suggest using M530 (contains M519 + 0.5 g/L MES) and add 10 g/L of sucrose.
A1375 Arabidopsis Germination Medium
Synonym: Arabidopsis seed germination medium
Properties
| Form: | Fine to Fluffy Powder |
| Appearance: | White to Yellow Powder |
| Application: | Arabidopsis seed germination |
| Solubility: | Water |
| Typical Working Concentration: | 12.67 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Murashige and Skoog (1962). pH = 3.5-4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 825 |
| Potassium Phosphate Monobasic | 85.0 |
| Potassium Nitrate | 950 |
| Zinc Sulfate 7H2O | 4.3 |
| Boric Acid | 3.1 |
| Calcium Chloride Anhydrous | 166.1 |
| Cobalt Chloride 6H2O | 0.0125 |
| MES | 500 |
| Cupric Sulfate 5H2O | 0.0125 |
| Sucrose | 10,000 |
| Na2 EDTA 2H2O | 18.63 |
| Ferrous Sulfate 7H2O | 13.9 |
| Magnesium Sulfate Anhydrous | 90.35 |
| Manganese Sulfate H2O | 8.45 |
| Sodium Molybdate 2H2O | 0.125 |
| Potassium Iodide | 0.415 |
Application Notes
Arabidopsis thaliana (Col-0) and (Ler) possess a relatively low level of seed dormancy, and it is most often broken by stratification (2-6°C) in the dark for 2-4 days.
This formulation contains 1/2X MS (M524) with 0.5 g/L MES, and 10 g/L of sucrose and meets what the ABRC (Arabidopsis Biological Resource Center) recommends (https://abrc.osu.edu/seed-handling) for Arabidopsis seed germination.
This formulation also contains enough nitrogen (20 mM in nitrate) and carbon (30 mM in sucrose) for proper seedling growth and development (Martin et al. 2002). As Arabidopsis thaliana is a model flowering plant, this type of formulation has been used often for germinating seeds (Denness et al. 2011; Stacey et al. 2006; Sorek et al. 2014).
If you prefer 1X MS for Arabidopsis germination, we would suggest using M530 (contains M519 + 0.5 g/L MES) and add 10 g/L of sucrose (Valvekens et al. 1988; Millet et al. 2010). To add Gamborg’s vitamins add 1 mL/L of G219.
References
Denness L, McKenna JF, Segonzac C, Wormit A, Madhou P, Bennett M, Mansfield J, Zipfel C, and Hamann T. (2011) Cell Wall Damage-Induced Lignin Biosynthesis Is Regulated by a Reactive Oxygen Species- and Jasmonic Acid-Dependent Process in Arabidopsis. Plant Physiol. Jul 2011; 156(3): 1364–1374.
Martin T, Oswald O, and Graham IA (2002) Arabidopsis Seedling Growth, Storage Lipid Mobilization, and Photosynthetic Gene Expression Are Regulated by Carbon:Nitrogen Availability. Plant Physiol. Vol. 128 (2) pg. 472-481
Stacey MG, Osawa H, Patel A, Gassmann W, and Stacey G (2006) Expression analyses of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction. Planta Vol. 223(2) pg. 291- 305
Sorek N, Sorek H, Kijac A, Szemenyei HJ, Bauer S, Hématy K, Wemmer DE, and Somerville CR (2014) The Arabidopsis COBRA protein facilitates cellulose crystallization at the plasma membrane. JBC First published online 20Oct2014 doi: 10.1074/jbc.M114.607192
Valvekens D, Van Montagu M, and Van Lijsebettens M. (1988) Agrobacterium tumefaciens- mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. PNAS 85, 5536-5540
Millet YA, Danna CH, Clay NK, Songnuan W, Simon MD, Werck-Reichhart D, and Ausubel FM (2010) Innate Immune Responses Activated in Arabidopsis Roots by Microbe-Associated Molecular Patterns. Plant Cell Vol. 22 pg. 973-990
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |