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Introduction:
Contains the macro- and micronutrients as described by Anderson (1978, 1980).
Anderson achieved a two-fold increase in multiplication of red raspberries using this formulation compared to MS. The optimal concentrations of growth regulators for shoot multiplication of red and black raspberries was 0.1 - 2.5 µM IBA and 4.5 - 9.0 µM 6-BA.
A267 Anderson Basal Salt Mixture
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 1.89 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Anderson (1978, 1980) pH = 3.25 – 4.25 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 400 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride·6H2O | 0.025 |
| Cupric Sulfate·5H2O | 0.025 |
| Na2 EDTA·2H2O | 74.5 |
| Ferrous Sulfate·7H2O | 55.7 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate·H2O | 16.9 |
| Molybdic Acid (Sodium Salt)·2H2O | 0.25 |
| Potassium Iodide | 0.3 |
| Potassium Nitrate | 480 |
| Sodium Phosphate Monobasic | 330.6 |
| Zinc Sulfate·7H2O | 8.6 |
Application Notes
Plant Tissue Culture Tested Plant Species: Rhododendron, Rubus (red and black raspberry).
Anderson achieved a two-fold increase in multiplication of red raspberries using this formulation compared to MS. The optimal concentrations of growth regulators for shoot multiplication of red and black raspberries was 0.1 – 2.5 µM IBA and 4.5 – 9.0 µM BA. Anderson’s medium contains approximately ¼ strength NH4NO3 and KNO3 compared to MS.
References
Anderson, WC. 1978. Tissue culture propagation of Rhododendrons. In Vitro 14: 334. Anderson, WC. 1980. Tissue culture propagation of red and black raspberries ,Rubus idaeus and R. occidentalis . Acta Hort. 112: 13-20.
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |