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Introduction:
6-Benzylaminopurine is the most widely used adenine-based cytokinin.
Synonyms: BA; N6-Benzyladenine
B130 6-Benzylaminopurine Solution (1 mg/mL)
| Synonyms: | BA; N6-Benzyladenine |
| CAS: | 1214-39-7 |
| Formula: | C12H11N5 |
| Mol. Weight: | 225.3 |
Properties
| Form: | Liquid |
| Appearance: | Colorless, Clear Liquid |
| Application: | Plant Growth Regulator; Cytokinin |
| Solubility: | Miscible with Water |
| Storage Temp: | 2 to 8 °C |
| Typical Working Concentration: | Varies by application. Concentration should be determined by end user |
| Other Notes: | Plant Tissue Culture Tested; For Research Use only |
Application Notes
6-Benzylaminopurine(BA) is one of the most popular cytokinins used to stimulate in vitro shoot development. It is often used in combination with an auxin, e.g., Indole-3-acetic acid (IAA), Napthaleneacetic acid (NAA), Indole-3- butyric acid (IBA).
BA is active across a broad range of plant species. Typical working concentration of BA is between 0.1 – 5.0 mg/L. It has been reported that 5-10 mg/L of BA is an optimal range for shoot multiplication of banana culture1 ; while 1.0 and 2.0 mg/L of BA have been used for blackberry culture. 2 At PhytoTechnology Laboratories® most cultures (e.g., Achimenes, African violet, ajuga, begonia, hosta, syngonium, etc.) are maintained on MS medium supplemented with 1 mg/L of BA and 0.025 mg/L of NAA.
PhytoTechnology Laboratories® also carried 6-Benzylaminopurine Powder, Product No. B800.Please Note: While PhytoTechnology Laboratories™ tests each lot of this product with two or more plant cell/ tissue culture lines, it is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.
References
1. Vuylsteke, D.R. 1998. Shoot-tip culture for the propagation, conservation, and distribution of Musa germplasm. International Institute of Tropical Agriculture, Ibadan, Nigeria. 82 pp.
2. Bobrowski, Vera L., Mello-Farias, Paulo C., and Peters, Jose A. 1996. Micropropagation of blackberries (Rubus sp.) cultivars. Rev. Bras. De Agrociencia. 2(1):17-20.
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |