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Introduction:
Contains the macro- and micronutrients, vitamins, and plant growth regulators required to culture orchids. Contains 0.2 mg/L 6-benzylaminopurine (BA).
B142 BM-2 Terrestrial Orchid Medium
| Form: | Powder |
| Appearance: | White to Cream |
| Application: | Plant Tissue Culture |
| Solubility: | Fully Soluble in Hot Water, Partially Soluble in Cold Water |
| Typical Working Concentration: | 27.22 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Same formulation as B141 with 0.2 mg/L BA; contains agar. Unadjusted pH = 5.0 – 6.0 BM-2 differs from BM Medium in that it (BM-2) has no inorganic nitrogen and it contains hydrolyzed casein, L-glutamine, and 6-Benzylaminopurine (BA). |
| Formula (mg/L) | |
|---|---|
| Boric Acid | 10 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2 EDTA.2H2O | 37.25 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 100 |
| Manganese Sulfate.H2O | 25 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
| Potassium Phosphate, Monobasic | 300 |
| Zinc Sulfate.7H2O | 10 |
| Agar | 6,000 |
| 6-Benzylaminopurine (BA) | 0.2 |
| D-Biotin | 0.05 |
| Casein, Enzymatic Hydrolysate | 500 |
| Folic Acid | 0.5 |
| L-Glutamine | 100 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 5 |
| Pyridoxine.HCl | 0.5 |
| Sucrose | 20,000 |
| Thiamine.HCl | 0.5 |
Application Notes
Plant Tissue Culture Tested
Especially suited for terrestrial orchids Paphiopedilum and Phragmipedium.
References
According to "The Orchid Seedbank Project" this was originally cited by Van Waes, 1984. In vitro studie van de kiemingsfysiologie van Westeuropese orchideeën. Thesis. Rijkuniversiteit Gent.