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Introduction:
A freshwater standard growth medium for cyanobacteria (e.g., Synechocytis spp. PCC 6803).
B1511 BG-11 Medium
| Synonyms: | Blue-Green (BG) Medium |
Properties
| Form: | Powder |
| Appearance: | Cream |
| Application: | Cyanobacteria Growth Medium |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 1.68 g/L |
| Storage Temp: | 2-8ºC |
| Storage Temp of Stock Solution: | 2-8ºC |
| Biological Assay: | Cell culture tested with Synechocystis sp. PCC 6803. |
| Formula (mg/L) | |
|---|---|
| Boric Acid | 2.86 |
| Manganese Chloride·4H2O | 1.81 |
| Calcium Chloride Anhydrous | 27.18 |
| Na2·Mg·EDTA | 1 |
| Citric Acid, Anhydrous | 6 |
| Potassium Phosphate dibasic | 40 |
| Cobalt Nitrate·6H2O | 0.049 |
| Sodium Carbonate Anhydrous | 20 |
| Cupric Sulfate·5H2O | 0.079 |
| Sodium Molybdate·2H2O | 0.39 |
| Ferric Ammonium Citrate | 6 |
| Sodium Nitrate | 1500 |
| Magnesium Sulfate·7H2O | 75 |
| Zinc Sulfate*7H2O | 0.222 |
Application Notes
BG-11 is generally a freshwater growth medium for culturing Cyanobacteria such as Synechocystis sp. PCC 6803.
HEPES - H326 (Akira et al. 1996) or TES (Xu et al. 1994) can be added to buffer the pH. After dissolving the powder medium, adjust to pH 8.0 with KOH or NaOH, and autoclave.
20 mM Hepes (H326) was used during biological testing of this product with Synechocystis sp. PCC 6803.
References
Akira, K., K. Lee, H. Fukuzawa, K. Ohyama, and T. Ogawa. (1996) cemA homologue essential to CO2 transport in the cynanobacterium Synechocystis PCC 6803. Proceedings of the National Academy of Sciences. 93:4006-4010.
Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1-61.
Stanier, R.Y., R. Kunisawa, M. Mandel, and G. Cohen-Bazire. (1971) "Purification and Properties of Unicellular Blue-Green Algae (Order Chroococcales)." Bacteriological Reviews 35(2):171-205.
Xu, H., D. Vavilin, C. Funk, and W. Vermaas. "Multiple Deletions of Small-Cab-like Proteins.