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Introduction:
Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965). Solution at a 50x concentration.
Requires 1 mL/L Sulfuric Acid, 0.1% (v/v) (Product # S7664).
B1650 Bold's Basal Medium, 50x
| Synonyms: | BBM |
Properties
| Properties | |
|---|---|
| Form: | Liquid |
| Appearance: | Clear with Light Green to Purple Tint |
| Application: | Freshwater algal culture |
| Solubility: | Miscible with Water |
| Typical Working Concentration: | 20.0 mL/L |
| Storage Temp: | 2-8ºC |
| Other Notes: | Approx. Solution pH is 4.2 +/- 0.5 It is suggested not to autoclave this product in its 50x concentrate form, as precipitation may result. Product may develop a purple tint over time. |
| Formula (mg/L) | |
|---|---|
| Boric Acid | 571 |
| Calcium Chloride, Anhydrous | 943.6 |
| Cobalt Nitrate•6H2O | 24.5 |
| Cupric Sulfate•5H2O | 78.5 |
| EDTA, Disodium Salt | 3180.5 |
| Ferrous Sulfate•7H2O | 249 |
| Magnesium Sulfate, Anhydrous | 1831.3 |
| Manganese Chloride•4H2O | 72 |
| Sodium Molybdate | 59.7 |
| Potassium Hydroxide | 1550 |
| Potassium Phosphate, Dibasic | 3750 |
| Potassium Phosphate, Monobasic | 8750 |
| Sodium Chloride | 1250 |
| Sodium Nitrate | 12500 |
| Zinc Sulfate.7H2O | 441 |
Application Notes
Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965).
Other algae species commonly grown in Bold's Basal Medium:
Zyngogonium ericetorum (Stancheva et al., 2014)
Media Preparation:
The standard medium is prepared as follows: Add 20.0 mL of the BBM 50x concentrate to ~0.98 liters of DI water.
The final solution pH is adjusted to 6.6 +/- 0.1 with KOH (Stein, 1973).
References
Brown, R.M., Larson, D.A., and H.C. Bold. (1964) Science 143(3606), 583-585.
Nichols H.W., and H.C. Bold (1965) J. Phycology 1, 34-38.
Stein J. (1973) Handbook of Phycological methods. Culture Methods and Growth Measurements. Cambridge
University Press. 448 pp