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Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965)
Requires 1 mL/L Sulfuric Acid, 0.1% (v/v) (Product # S7664).
Synonyms: | BBM |
Properties | |
---|---|
Form: | Powder |
Appearance: | Off-White to Gray/Green |
Application: | Freshwater algal culture |
Solubility: | Soluble in Water |
Typical Working Concentration: | 0.705 g/L |
Storage Temp: | 2-8ºC |
Storage Temp of Stock Solution: | 2-8ºC |
Other Notes: | In order to conform with the original reference, 1.0 mL of 0.1% Sulfuric Acid Solution should be added per liter of this medium when prepared at a 1x concentration. Product or solutions may develop a purple tint over time. |
Formula (mg/L) | |
---|---|
Boric Acid | 11.42 |
Manganese Chloride.4H2O | 1.44 |
Calcium Chloride, Anhydrous | 18.87 |
Potassium Hydroxide | 31 |
Cobalt Nitrate.6H2O | 0.49 |
Potassium Phosphate, Dibasic | 75 |
Cupric Sulfate.5H2O | 1.57 |
Potassium Phosphate, Monobasic | 175 |
EDTA, Disodium Salt | 63.61 |
Sodium Chloride | 25 |
Ferrous Sulfate.7H2O | 4.98 |
Sodium Molybdate | 1.19 |
Magnesium Sulfate, Anhydrous | 36.63 |
Sodium Nitrate | 250 |
Zinc Sulfate.7H2O | 8.82 |
Application Notes
Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965).
Other algae species commonly grown in Bold's Basal Medium:
Zyngogonium ericetorum (Stancheva et al., 2014)
Media Preparation:
The standard medium is prepared as follows: Add 1.0 mL of 0.1% Sulfuric Acid solution to 1 liter of DI water along with 0.705 g of B1675. The final solution pH is adjusted to 6.6 +/- 0.1 with KOH (Stein, 1973).
References
Brown, R.M., Larson, D.A., and H.C. Bold. (1964) Science 143(3606), 583-585.
Nichols H.W., and H.C. Bold (1965) J. Phycology 1, 34-38.
Stancheva, R., Hall, J. D., Herburger, K., Lewis, L. A., McCourt, R. M., Sheath, R. G., & Holzinger, A. (2014).
Phylogenetic position of Zygogonium ericetorum (Zygnematophyceae, Charophyta) from a high alpine habitat and ultrastructural characterization of unusual aplanospores. Journal of Phycology, 50(5), 790-803.
Stein J. (1973) Handbook of Phycological methods. Culture Methods and Growth Measurements. Cambridge University Press. 448 pp.
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |