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Introduction:
Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965)
Requires 1 mL/L Sulfuric Acid, 0.1% (v/v) (Product # S7664).
B1675 Bold's Basal Medium
| Synonyms: | BBM |
Properties
| Properties | |
|---|---|
| Form: | Powder |
| Appearance: | Off-White to Gray/Green |
| Application: | Freshwater algal culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 0.705 g/L |
| Storage Temp: | 2-8ºC |
| Storage Temp of Stock Solution: | 2-8ºC |
| Other Notes: | In order to conform with the original reference, 1.0 mL of 0.1% Sulfuric Acid Solution should be added per liter of this medium when prepared at a 1x concentration. Product or solutions may develop a purple tint over time. |
| Formula (mg/L) | |
|---|---|
| Boric Acid | 11.42 |
| Manganese Chloride.4H2O | 1.44 |
| Calcium Chloride, Anhydrous | 18.87 |
| Potassium Hydroxide | 31 |
| Cobalt Nitrate.6H2O | 0.49 |
| Potassium Phosphate, Dibasic | 75 |
| Cupric Sulfate.5H2O | 1.57 |
| Potassium Phosphate, Monobasic | 175 |
| EDTA, Disodium Salt | 63.61 |
| Sodium Chloride | 25 |
| Ferrous Sulfate.7H2O | 4.98 |
| Sodium Molybdate | 1.19 |
| Magnesium Sulfate, Anhydrous | 36.63 |
| Sodium Nitrate | 250 |
| Zinc Sulfate.7H2O | 8.82 |
Application Notes
Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965).
Other algae species commonly grown in Bold's Basal Medium:
Zyngogonium ericetorum (Stancheva et al., 2014)
Media Preparation:
The standard medium is prepared as follows: Add 1.0 mL of 0.1% Sulfuric Acid solution to 1 liter of DI water along with 0.705 g of B1675. The final solution pH is adjusted to 6.6 +/- 0.1 with KOH (Stein, 1973).
References
Brown, R.M., Larson, D.A., and H.C. Bold. (1964) Science 143(3606), 583-585.
Nichols H.W., and H.C. Bold (1965) J. Phycology 1, 34-38.
Stancheva, R., Hall, J. D., Herburger, K., Lewis, L. A., McCourt, R. M., Sheath, R. G., & Holzinger, A. (2014).
Phylogenetic position of Zygogonium ericetorum (Zygnematophyceae, Charophyta) from a high alpine habitat and ultrastructural characterization of unusual aplanospores. Journal of Phycology, 50(5), 790-803.
Stein J. (1973) Handbook of Phycological methods. Culture Methods and Growth Measurements. Cambridge University Press. 448 pp.