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Introduction:
Contains the macro-and micronutrients, vitamins, sucrose fructose, agar, and supplements required to culture terrestrial orchids.
B470 BM-2 Terrestrial Orchid Medium w/out Agar
| Properties | |
|---|---|
| Form: | Powder |
| Appearance: | White to Cream |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 21.22 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Same formulation as B141 with 0.2 mg/L BA Unadjusted pH = 5.0 – 6.0 BM-2 differs from BM Medium in that it (BM-2) has no inorganic nitrogen and it contains hydrolyzed casein, L-glutamine and BA. |
| Formula (mg/L) | |
|---|---|
| Boric Acid | 10 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2 EDTA.2H2O | 37.25 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 100 |
| Manganese Sulfate.H2O | 25 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
| Potassium Phosphate, Monobasic | 300 |
| Zinc Sulfate.7H2O | 10 |
| 6-Benzylaminopurine (BA) | 0.2 |
| D-Biotin | 0.05 |
| Casein, Enzymatic Hydrolysate | 500 |
| Folic Acid | 0.5 |
| L-Glutamine | 100 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 5 |
| Pyridoxine.HCl | 0.5 |
| Sucrose | 20,000 |
| Thiamine.HCl | 0.5 |
Application Notes
Plant Tissue Culture Tested
Well suited for the terrestrial orchids Paphiopedilum and Phragmipedium.
References
According to "The Orchid Seedbank Project" this was originally cited by Van Waes, 1984. In vitro studie van de kiemingsfysiologie van Westeuropese orchideeën. Thesis. Rijkuniversiteit Gent.