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Introduction:
B514 Blaydes Modified Basal Medium
| Form: | Powder |
| Appearance: | Off-white to yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 31.86 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients, sucrose, and thiamine as described by Blaydes (1966). Plant Tissue Culture Tested. |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1000 |
| Boric Acid | 1.6 |
| Calcium Nitrate | 241.1 |
| Na2EDTA.2H2O | 74.5 |
| Ferrous Sulfate.7H2O | 55.7 |
| Magnesium Sulfate, Anhydrous | 17.1 |
| Manganese Sulfate.H2O | 4.4 |
| Potassium Chloride | 65 |
| Potassium Iodide | 0.8 |
| Potassium Nitrate | 100 |
| Potassium Phosphate, Monobasic | 300 |
| Zinc Sulfate.7H2O | 1.5 |
| Glycine (Free Base) | 2 |
| Sucrose | 30,000 |
| Thiamine.HCl | 0.1 |
Application Notes
Plant species: Alfalfa
Callus initiation was achieved when the basal medium was supplemented with 1.9 mg/L Kinetin and 8.0 – 19.0 mg/L 2,4-D. Regeneration was achieved when the Kinetin and 2,4-D were omitted and the basal medium was supplemented with 100 mg/L myo-Inositol and 2,000 mg/L yeast extract
References
Blaydes, OF. 1966. Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol. Plant 19: 748-753.