Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
A carbon source and substrate for ß-glucosidase.
Can be used in mD5A Medium (Prod. # M5516) for the selection of Xanthomonas hortorum. Also used in Cefazoline-Cellobiose-Methionine (CCM) medium as a carbon source for Xanthomonas axonopodis pv. vignicola.Synonyms: 4-O-ß-D-Glucopyranosyl-D-glucose
Synonyms: | 4-O-β-D-Glucopyranosyl-D-glucose |
CAS: | 528-50-7 |
Formula: | C12H22O11 |
Mol. Weight: | 342.30 |
Form: | Powder |
Appearance: | White to Off-White |
Application: | Microbiology, Seed Testing, Phytopathology |
Solubility: | Soluble in Water |
Typical Working Concentration: | Varies with application, should be determined by end user. |
Storage Temp: | Room Temperature |
Product# | Product Name | Amount/L |
P705 | Dipotassium Phosphate, (K2HPO4) | 1.34 g/L |
P846 | Monopotassium Phosphate (KH2PO4) | 0.4 g/L |
M150 | Magnesium Sulfate (Anhydrous) (MgSO4) | 0.3 g/L |
B210 | Boric Acid (H3BO3) | 0.2 g/L |
A109 | Ammonium Chloride | 1.0 g/L |
C1842 | Cellobiose | 10.0 g/L |
M539 | Methionine | 1.0 g/L |
A111/A296 | Agar | 14.0 g/L |
C1989/C1796 | *Cycloheximide Powder/ Solution (100 mg/mL) | 0.2 g/L or 2.0 mL/L |
N/A | *Cefazoline | 0.010 g/L |
Adjust to pH 7.2 Add aseptically after autoclaving medium and allowing to cool. |
Application Notes
Cellobiose is used in microbiological and phytopathological media as a carbon source for selection of specific microbes. It is used in multiple selection media, including mD5A Medium (Prod # M5516) and CCM medium. The table below is an example of the use of cellobiose as a carbon source in a selection medium.
The semi-selective medium designated as Cefazoline-Cellobiose-Methionine (CCM) medium is intended to select for Xanthomonas axonopodis pv. vignicola and contains the following components per liter (Wydra et al., 2004):
References
Wydra K, G Khatri-Chhetri, A Mavridis, R Sikirou, K Rudolph (2004) A diagnostic medium for the semi-selective
isolation and enumeration of Xanthomonas axonopodis pv. vignicola. European Journal of Plant Pathology,110(10) pp. 991-1001.
Formula (mg/L) | |
---|---|
Boric Acid | 143 |
Calcium Chloride Anhydrous | 1359 |
Citric Acid, Anhydrous | 300 |
Cobalt Nitrate·6H2O | 2.45 |
Cupric Sulfate∙5H2O | 3.95 |
Ferric Ammonium Citrate | 300 |
Magnesium Sulfate∙7H2O | 3750 |
Manganese Chloride∙4H2O | 90.5 |
Na2∙Mg∙EDTA | 50 |
Potassium Phosphate dibasic | 2000 |
Sodium Carbonate Anhydrous | 1000 |
Sodium Molybdate∙2H2O | 19.5 |
Sodium Nitrate | 75000 |
Zinc Sulfate*7H2O | 0.222 |
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |