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Introduction:
Coconut water is a natural, no additive supplement of mainly vitamins and amino acids for plant tissue culture.
C195 Coconut Water
Properties
| Form: | Liquid |
| Appearance: | Clear, Colorless to Slight Yellow Tint |
| Application: | Plant Media Supplement |
| Solubility: | Miscible with Water |
| Typical Working Concentration: | 25 to 50 mL/L |
| Storage Temp: | 2 to 8 °C |
| Other Notes: | Plant Tissue Culture Tested Sterile Filtere |
Application Notes
Coconut water has been shown to stimulate shoot proliferation in man
y plant species
. This is most likely due to its
unique composition of nutrients and natural phytohormones (e.g. zeatin and kinetin)
(Mamaril
et al
., 1988).
It is
most commonly used in orchid tissue culture as a medium supplement. Introduced by Overbeek et al. in 1940,
coconut water was used as a new component of nutrient medium for callus cultures (1941) and has since become
a popular additive
in tissue culture media.
PhytoTechnology Laboratories® also carries Coconut Powder, Product No. C187, and Freeze Dried Organic Coconut Powder, Product No. C1780.
References
Afroz A, Z. Chaudhry, U. Rashid, M.R. Khan and G.M. Ali (2010) Enhanced regeneration in explants of tomato (Lycopersicon esculentum L.) with the treatment of coconut water. African Journal of Biotechnology 9(24), pp. 3634-
3644.
Alam MF, Sinha P, Hakim ML.(2010) Micropropagation of
Vanda teres
(Roxb.) Lindle.
Methods Mol Biol
. 589:21-
8.
Al-Khayri, JM (2010) Somatic embryogenesis of date palm (
Phoenix dactylifera
L.) improved by coconut water.
Biotechnology
, 9: 477-
484.
DOI:
10.3923/biotech.2010.477.484
Baskaran, P and N. Jayabalan (2005) An Efficient Micropropagation System for Eclipta alba: A Valuable Medicinal
Herb.
In Vitro Cellular & Developmental Biology
.
Plant,
41:4 Pp. 532-
539.
Jayasih, Y. and Wattimena, D.I.G. (1994) The effect of coconut water and zeolite on
micropropagation of potato
shoot.
Acta Hort.
(ISHS) 369:451
-454
http://www.actahort.org/books/369/369_49.htm
Kunisaki J, Araki A, Sagawa Y (2003) Micropropagation of 'awa (kava,
Piper methysticum
). Honolulu (HI):
University of Hawaii. 11 p.
Biotechnology
; BIO
-4.
http://hdl.handle.net/10125/2842
Mandal AB, A. Maiti and Elanchezhian R. (2002) In vitro micropropagation of
Ananas comosus
L. (Merr.) var
.
Queen.
Jour. Appl. Hort
. 4(2):107-
112.
Martin KP (2007) Micropropagation of the bamboo orchid (
Arundina graminifolia
(d. Don) hochr.) through
protocorm
-like
-bodies using node explants.
Propagation of Ornamental Plants
7(2): 97-
100.
Modi, P.; Banerjee, M.
(2009) Effect of growth regulator and additives (AdSO4, zeatin and coconut water) on
micropropagation of
Rauvolfia serpentina
.
Journal of Applied Bioscience
, v. 35(1): p. 79-
81.
Nasib A, K. Ali and S. Khan (2008) An optimized and improved method for the i
n vitro propagation of kiwifruit
(
Actinidia deliciosa
) using coconut water.
Pak. J. Bot
., 40(6): 2355-
2360.
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |