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Introduction:
Contains the macro- and micronutrients as described by Chee & Pool (1987).
This medium was developed to improve grape shoot multiplication. This formulation has reduced levels of Chloride, Iodide, and manganese compared to MS. Calcium Chloride was replaced by Calcium Nitrate, thus improving the quality of grape shoots.
C287 Chee & Pool C2d Vitis Basal Medium
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water Soluble |
| Typical Working Concentration: | 4.49g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Chee and Pool (1987). pH = 3.5 – 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Nitrate | 492.3 |
| Cobalt Chloride·6H2O | 0.025 |
| Cupric Sulfate·5H2O | 0.025 |
| Na2 EDTA·2H2O | 37.3 |
| Ferrous Sulfate·7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 180.6 |
| Manganese Sulfate·H2O | 0.845 |
| Molybdic Acid (Sodium Salt)·2H2O | 0.25 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate·7H2O | 8.6 |
| myo-Inositol | 10 |
| Nicotinic Acid (Free Acid) | 1.0 |
| Pyridoxine·HCl | 1.0 |
| Thiamine·HCl | 1.0 |
Application Notes
Plant Tissue Culture Tested
Plant Species: Grape (Vitis spp) This medium was developed to improve grape shoot multiplication. This formulation has reduced levels of chloride, Iodide, and manganese compared to MS. Calcium chloride was replaced by calcium nitrate, thus improving the quality of grape shoots.
References
Chee, R and RM Pool. 1987. Improved inorganic media constituents for in vitro shoot multiplication of Vitis. Sci. Hort. 32: 85.