 |
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
Introduction:
Carbenicillin is a derivative of penicillin with a mode of action similar to benzylpenicillin. It is the most commonly used antibiotic for the elimination of Argobacterium tumefaciens during plant transformation.
C346 Carbenicillin, Disodium Salt
| Synonym: | a - Carboxybenzylpenicillin, Disodium Salt |
| CAS: | 4800 - 94 - 6 |
| Formula: | C 17 H 16 N 2 O 6 SNa 2 |
| Molecular Wt: | 422.41 |
Properties
| Properties | |
| Form: | Powder |
| Appearance: | White to Cream Powder |
| Application: | Plant Tissue Cultur e Antibiotic |
| Solubility: | Water in Water |
| Typical Working Concentration: | 2 to 6°C |
| Storage Temp: | 2 to 8 °C |
| Storage Temp of Stock Solution: | 2 to 8°C |
| Other Notes: | Stock solution is stable for 24 hrs when stored at Room Temperature, 72 hrs at 2 - 6 o C and 12 months at - 20 o C (i.e. non - frost freezer) |
Application Notes
Carbenicillin is a derivative
of penicillin with
a
mode of action similar to benzylpenicillin. It is the most
commonly used antibiotic for the elimination of
Argobacterium
tumefaciens
due to its relatively low
toxicity for
a wide range of plant sp
ecies
. A concentration of 500 mg/
L is recommended to achieve
microbe toxicity; however, concentration
s up to 1000 mg/L have been reported
in plant tissue culture
literature.
Please Note: While
Phyto
Technology Laboratories® tests each lot of this product with
two or more plant
cell/ tissue culture lines, it is the sole responsibility of the purchaser to determine the appropriateness of
this product for the specific plants that are being cultured and applications that are being used.
References
Merck 13, 1801
Sweetman SC (ed)
(2007)
,
Martindale: The Complete Drug Reference
35. C
hina: Pharmaceutical Press
.
Nauerby
B,
Billing
K
,
and
Wyndaele
R. (1997)
Influence of the antibiotic timentin on plant regeneration
compared to carbenicillin and cefotaxime in concentr
ations suitable for elimination of
Agrobacterium tumefaciens
. Plant Science Vol. 123(1
-
2) pg. 169
-
177.