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Introduction:
Contains macro- and micronutrients as described by Gupta & Durzan (1985).
This medium was developed to promote shoot proliferation. This formulation has approximately ¼ the concentration of NH4NO3 and KNO3 compared to MS. Additional Ca+2 and NO3- ions are supplied by Ca(NO3)2.
D146 DCR Basal Salt Mixture
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 1.64g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Chu et al. (1975). pH = 3.5 – 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 400 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 64.14 |
| Calcium Nitrate | 386.31 |
| Cobalt Chloride·6H2O | 0.025 |
| Cupric Sulfate·5H2O | 0.25 |
| Na2 EDTA·2H2O | 37.3 |
| Ferrous Sulfate·7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate·H2O | 22.3 |
| Molybdic Acid (Sodium Salt)·2H2O | 0.25 |
| Nickel Chloride·6H2O | 0.025 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 340 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate·7H2O | 8.6 |
Application Notes
Plant Tissue Culture Tested
Plant Species: Douglas Fir (Pseudotsuga spp.) and Sugar Pine (Pinus lambertiana)
This medium was developed to promote shoot proliferation. This formulation has approximately ¼ the concentration of NH4NO3 and KNO3 compared to MS. Additional Ca+2 and NO3 - ions are supplied by Ca(NO3)2.
References
Gupta, PK and KJ Durzan. 1985. Shoot multiplication from mature Douglas-fir and sugar pine. Plant Cell Reports 4:177-179.