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Introduction:
Contains the macro- and micronutrients and vitamins as described by De Greef and Jacobs (1979).
D2206 DE GREEF & JACOBS BASAL MEDIUM
Properties
| Form: | Powder |
| Appearance: | White to Light Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 3.91g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by De Greef & Jacobs (1979). Plant Tissue Culture Tested Unadjusted pH (approximately): 4.0 – 5.0 |
| Formula (mg/L) | |
|---|---|
| Ammonium Sulfate | 400.0 |
| Potassium Iodide | 1.58 |
| Boric Acid | 10.62 |
| Potassium Nitrate | 2,000 |
| Calcium Chloride, Anhydrous | 226.5 |
| Sodium Phosphate, Monobasic | 250.0 |
| Cobalt Chloride | 0.0025 |
| Sodium Molybdate | 0.0025 |
| Cupric Sulfate | 0.0025 |
| Zinc Sulfate 7H2O | 1.06 |
| Ferrous Sulfate 7H2O | 27.8 |
| myo-Inositol | 100.0 |
| Magnesium Sulfate Anhydrous | 244.33 |
| Nicotinic Acid | 1.0 |
| Manganese Sulfate H2O | 1.68 |
| Pyridoxine HCl | 1.0 |
| Na2 EDTA 2H2O | 37.26 |
| Thiamine HCl | 10.0 |
| Potassium Chloride | 600.0 |
Application Notes
Originally used in the tissue culture of sugar beets.
References
De Greef W, Jacobs M. 1979. In vitro culture of sugar beet: description of a cell line with high regeneration capacity. Plant Science Letters 17, 55-61.