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Introduction:
Contains the macro- and micronutrients and vitamins as described by Economou & Read (1984).
E575 Economou and Read Basal Medium
Properties
| Form: | Powder |
| Appearance: | Off-White to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 1.84g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Economou & Read (1984). Plant Tissue Culture Tested |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 400 |
| Ammonium Sulfate | 132 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Ferric Sodium DTPA | 56 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt). 2H2O | 0.25 |
| Potassium Nitrate | 202 |
| Potassium Phosphate, Monobasic | 408 |
| Zinc Sulfate.7H2O | 8.6 |
| myo-Inositol | 100 |
| Thiamine.HCl | 0.4 |
Application Notes
Plant species: Rhododendron spp. (Economou, 1984); Vaccinium vitis-idaea (Hosier et al., 1985)
This medium was originally developed for northern-hardy, deciduous azaleas.
References
Economou, AS and PE Read (1984) In vitro shoot proliferation of Minnesota deciduous azaleas, HortSci. 19(1):60-61. Hosier MA, Flatebo G, Read PE (1985) In vitro propagation of lingonberry. HortSci, 20: 364-365.