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Introduction:
Axenic maintenance medium for non-diatom marine phytoplankton, for example Dunaliella salina.
Formulation based on literature published by Guillard & Ryther (1962).
Intended to be dissolved in sea water
1 KG = Approx. 11,100 L or 2,930 gallons
10 KG = Approx. 111,000 L or 29,300 gallons
25 KG = Approx. 277,700 L or 73,360 gallons
G3454 f/2 Guillard’s Marine Enriched Seawater w/o Silicate
| Synonyms: | f/2 w/o Si |
Properties
| Form: | Fine to Coarse Powder |
| Appearance: | Cream to light yellow |
| Application: | Marine phytoplankton culture |
| Solubility: | Soluble in Seawater |
| Typical Working Concentration: | 0.09 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | 2-8°C |
| Biological Assay: | Biologically tested with Dunaliella salina |
| Formula (mg/L) | |
|---|---|
| Sodium Nitrate | 75.0 |
| Cobalt Chloride•6H2O | 0.01 |
| Cupric Sulfate•5H2O | 0.01 |
| EDTA, disodium salt | 4.36 |
| Ferric Chloride, Anhydrous | 1.90 |
| Manganese Chloride•4H2O | 0.18 |
| Molybdic Acid, disodium salt | 0.006 |
| Sodium Phosphate, Monobasic | 5.00 |
| Zinc Sulfate•7H2O | 0.022 |
| Biotin | 0.0005 |
| Vitamin B12 | 0.0005 |
| Thiamine HCl | 0.10 |
Application Notes
f/2 Guillard’s Marine Enriched Seawater is a powder formulation of the vitamins, macro- and trace-element solutions to be dissolved in seawater (S7650). This formulation utilizes EDTA and Ferric Chloride in molar equivalents (NCMA, Guillard and Ryther 1962). This medium DOES NOT contain silicate, so it can be used to for axenic maintenance of NON-diatom marine phytoplankton.
Species documented to be grown in f/2 without silicate: Symbiodinium (Mays & Kempf, 2014)
To make 1L of medium: Dissolve 0.10 g in 0.99L of seawater (S7650) and adjust the pH to the desired range using HCl or KOH. The original formulation, according to Guillard, used a pH of 7.2 +/- 0.1 (Guillard, 1962). Bring the final volume to 1.0L with seawater and autoclave the medium.
References
Guillard, R.R.L, and J.H. Ryther (1962) Studies of Marine Planktonic Diatoms: I. Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran. Canadian Journal of Microbiology 8, 229-239. Mays, M.M. & Kempf, S. C. (2014). Antigenic variation in mucilage secreted by members of the genus Symbiodinium (Dinophyceae). Journal of Phycology, 50(5), 850-859. NCMA. Provasoli-Guillard, National Center for Marine Algae and Microbiota. Available: https://ncma.bigelow.org/node/79