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Introduction:
A modification of the macro- and micronutrients of Murashige and Skoog Basal Medium with vitamins, PGRs, Sucrose and Agar.
| Form: | Powder |
| Appearance: | White to Off-White |
| Application: | Plant Tissue Culture |
| Solubility: | Partially Soluble in Water |
| Typical Working Concentration: | 43.40 g/L |
| Storage Temp: | 2-8°C |
| Other Notes: | Plant Tissue Culture Tested |
| Formula (mg/L) | |
|---|---|
| 6-Benzylaminopurine | 2 |
| Adenine Hemisulfate | 160 |
| Agar | 8000 |
| Ammonium Nitrate | 1650 |
| a-Napththaleneacetic Acid | 0.02 |
| Boric Acid | 6.2 |
| Calcium Chloride Anhydrous | 332.2 |
| Casein Hydrolysate | 500 |
| Cobalt Chloride Hexahydrate | 0.025 |
| Cupric Sulfate Pentahydrate | 0.025 |
| Ferrous Sulfate Heptahydrate | 27.8 |
| Glycine | 2 |
| Magnesium Sulfate Anhydrous | 180.7 |
| Magnesium Sulfate Monohydrate | 16.9 |
| Molybdic Acid, Sodium Salt | 0.25 |
| Myo-Inositol | 100 |
| Na2 EDTA Dihydrate | 37.26 |
| Potassium Iodide | 0.83 |
| Potassium Iodide | 1900 |
| Potassium Phosphate Monobasic | 300 |
| Sodium Phosphate Monobasic | 170 |
| Sucrose | 30000 |
| Thiamine HCl | 0.4 |
| Zinc Sulfate, Heptahydrate | 8.6 |
Application Notes
Same formulation as medium H436, except this formulation has a reduced amount of NAA.