Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
Stage III medium. Modified Murashige & Skoog (1962) with (mg/L): 0.1 6-BA, 500.0 Casein Hydrolysate, and 0.5 NAA.
Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and the vitamins described by Linsmaier & Skoog (1965). Also contains sucrose and agar.
Form: | Powder |
Appearance: | White to Yellow |
Application: | Plant Tissue Culture |
Solubility: | Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water. |
Typical Working Concentration: | 43.23 g/L |
Storage Temp: | 2-8°C |
Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: | Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains agar and sucrose. Plant Tissue Culture Tested. |
Formula (mg/L) | |
---|---|
Ammonium Nitrate | 1650 |
Boric Acid | 6.2 |
Calcium Chloride, Anhydrous | 332.2 |
Cobalt Chloride•6H2O | 0.025 |
Cupric Sulfate•5H2O | 0.025 |
Disodium EDTA •2H2O | 37.26 |
Ferrous Sulfate•7H2O | 27.8 |
Magnesium Sulfate, Anhydrous | 180.7 |
Manganese Sulfate•H2O | 16.9 |
Molybdic Acid (Sodium Salt)• 2H2O | 0.25 |
Potassium Iodide | 0.83 |
Potassium Nitrate | 1900 |
Potassium Phosphate, Monobasic | 300 |
Sodium Phosphate, Monobasic | 170 |
Zinc Sulfate•7H2O | 8.6 |
Agar | 8000 |
6-Benzylaminopurine (BA) | 0.1 |
Casein, Enzymatic Hydrolysate | 500 |
myo-Inositol | 100 |
α-Naphthaleneacetic Acid | 0.5 |
Sucrose | 30,000 |
Thiamine•HCl | 0.4 |
Application Notes
Plant species: Hosta
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127. Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |