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Introduction:
Contains the macro- and micronutrients as described by Kao & Michayluk (1975).
This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/mL) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. (See K427) Cell growth and division could also be enhanced by raising the concentrations of Calcium Chloride from 1 to 5 mM.
| Form: | Powder |
| Appearance: | White to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 3.65g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro and micronutrients as described by Kao & Michayluk (1975). |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 600 |
| Boric Acid | 3 |
| Calcium Chloride, Anhydrous | 453 |
| Cobalt Chloride·6H2O | 0.025 |
| Cupric Sulfate·5H2O | 0.025 |
| Na2 EDTA·2H2O | 37.26 |
| Ferrous Sulfate·7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 146.55 |
| Manganese Sulfate·H2O | 10 |
| Molybdic Acid (Sodium Salt)·2H2O | 0.25 |
| Potassium Chloride | 300 |
| Potassium Iodide | 0.75 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate·7H2O | 2 |
Application Notes
Plant species: Vicia hajastana (Kao & Michayluk, 1975); Vicia faba (Binding & Nehls, 1978); Daucus carota (Grzebelus et al, 2012).
This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/ml) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. (See K427) Cell growth and division could also be enhanced by raising the concentrations of calcium chloride from 1 to 5 mM.
References
Binding, H. and Nehls, R. (1978) Regeneration of Isolated Protoplasts of Vicia faba L. Z. Pflanzenpbysiol. 88, 327-332. Grzebelus E, M Szklarczyk & R Baranski (2012)
An improved protocol for plant regeneration from leafand hypocotyl-derived protoplasts of carrot. Plant Cell Tiss Organ Cult 109:101-109. Kao, KN and MR Michayluk (1975)
Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.