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Introduction:
Kao & Michayluk vitamin solution (100x).
Use at a concentration of 10 mL/L of medium to achieve the proper final concentration.
| Form: | Liquid |
| Appearance: | Clear, Colorless Solution |
| Application: | Plant Tissue Culture |
| Solubility: | Miscible with Water |
| Typical Working Concentration: | 10.0 mL/L of Medium |
| Storage Temp: | --20-0 °C |
| Other Notes: | Contains the vitamins as described by Kao and Michayluk (1975). Sterile Filtered. |
| Formula (mg/L) | |
|---|---|
| p-Aminobenzoic Acid | 2 |
| myo-Inositol | 10,000 |
| L-Ascorbic Acid | 200 |
| Niacinamide | 100 |
| D-Biotin | 1 |
| Pyridoxine.HCl | 100 |
| D-Calcium Pantothenate | 100 |
| Retinol; Vitamin A | 1 |
| Choline Chloride | 100 |
| Riboflavin | 20 |
| Cyancobalamin; Vit B12 | 2 |
| Thiamine.HCl | 100 |
Application Notes
Plant species: Vicia hajastana (Kao & Michayluk, 1975); Vicia faba (Binding & Nehls, 1978);
Daucus carota (Grzebelus et al, 2012).
References
Binding, H. and Nehls, R. (1978) Regeneration of Isolated Protoplasts of Vicia faba L. Z. Pflanzenpbysiol. 88, 327-332.
Grzebelus E, M Szklarczyk & R Baranski (2012) An improved protocol for plant regeneration from leafand hypocotyl-derived protoplasts of carrot. Plant Cell Tiss Organ Cult 109:101-109.
Kao, KN and MR Michayluk (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.