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Introduction:
Non-selective general bacterial subculturing medium.
Often used for the detection & culture of pseudomonads such as Pseudomonas aeruginosa and Psuedomonas syringae by the addition of cephalexin & nystatin (see product info sheet for details) and for distinguishing fluorescent pseudomonads from nonfluorescent pseudomonads.
10 mL/L of Glycerol (G381) should be added to the medium before autoclaving.
K5013 King’s B Medium
| Synonyms: | KB Medium, KB Agar, King’s Agar B, King’s Base Medium; KBM; KB; King's B Agar; Pseudomonas Agar F |
Properties
| Form: | Powder |
| Appearance: | Off-White to Tan |
| Application: | Phytopathology, Microbiology, Seed Testing, Plant Defense & Immunity |
| Solubility: | Partially Soluble in Cold Water, Soluble in Boiling Water |
| Typical Working Concentration: | 37.23 g/L |
| Storage Temp: | Room Temperature |
| Other Notes: | Add 10-15 mL/L of Glycerol (Prod # G381) prior to autoclaving for carbon source. Add 50 mg Cephalexin (Prod # C2112 or C1970) and 35 mg Nystatin (Prod # N581) per liter to select for Pseudomonads (Vogel & Bonner, 1956) |