Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981).
This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium Nitrate has been eliminated from this medium and replaced with Potassium Sulfate.
Synonym: WPM
Form: | Powder |
Appearance: | White to Yellow Powder |
Application: | Plant Tissue Culture |
Solubility: | Water |
Typical Working Concentration: | 2.41g/L |
Storage Temp: | 2-8°C |
Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: | Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5 |
Formula (mg/L) | |
---|---|
Ammonium Nitrate | 400 |
Boric Acid | 6.2 |
Calcium Chloride, Anhydrous | 72.5 |
Calcium Nitrate | 386 |
Cupric Sulfate.5H2O | 0.25 |
Na2 EDTA.2H2O | 37.3 |
Ferrous Sulfate.7H2O | 27.85 |
Magnesium Sulfate, Anhydrous | 180.7 |
Manganese Sulfate.H2O | 22.3 |
Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
Potassium Phosphate, Monobasic | 170 |
Potassium Sulfate | 990 |
Zinc Sulfate.7H2O | 8.6 |
Glycine (Free Base) | 2 |
myo-Inositol | 100 |
Nicotinic Acid (Free Acid) | 0.5 |
Pyridoxine.HCl | 0.5 |
Thiamine.HCl | 1 |
Application Notes
Plant Tissue Culture Tested
Plant species: Kalmia latifolia (Mountain Laurel), Rhododendron spp. This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium nitrate has been eliminated from this medium and replaced with potassium sulfate. The levels of ammonium nitrate are ¼x compared to MS. Nitrogen is also provided by calcium nitrate.
References
Lloyd, G and BH McCown. 1981. Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture. Proc. Int. Plant Prop. Soc. 30:421-427.
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |