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Introduction:
Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981).
This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium Nitrate has been eliminated from this medium and replaced with Potassium Sulfate.
L449 Lloyd & McCown Woody Plant Basal Medium
Synonym: WPM
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 2.41g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 400 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 72.5 |
| Calcium Nitrate | 386 |
| Cupric Sulfate.5H2O | 0.25 |
| Na2 EDTA.2H2O | 37.3 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 22.3 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
| Potassium Phosphate, Monobasic | 170 |
| Potassium Sulfate | 990 |
| Zinc Sulfate.7H2O | 8.6 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 0.5 |
| Pyridoxine.HCl | 0.5 |
| Thiamine.HCl | 1 |
Application Notes
Plant Tissue Culture Tested
Plant species: Kalmia latifolia (Mountain Laurel), Rhododendron spp. This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium nitrate has been eliminated from this medium and replaced with potassium sulfate. The levels of ammonium nitrate are ¼x compared to MS. Nitrogen is also provided by calcium nitrate.
References
Lloyd, G and BH McCown. 1981. Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture. Proc. Int. Plant Prop. Soc. 30:421-427.