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Introduction:
Contains the macro-& micronutrients, & vitamins as described by L & S (1964). With 30 g/L sucrose & 7 g/L agar. pH adjusted and buffered.
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR's, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuation during media preparation and culture.
L452 Linsmaier & Skoog Modified Basal Medium w/ 30 g/L Sucrose & 7 g/L Agar pH Adjusted and Buffered
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)
Properties
| Form: | Powder |
| Appearance: | White to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 42.53 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Linsmaier and Skoog (1965) |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2 EDTA.2H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
| Potassium Hydroxide | 100 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| Agar | 7000 |
| MES (Free Acid) | 1000 |
| myo-Inositol | 100 |
| Sucrose | 30,000 |
| Thiamine.HCl | 0.4 |
Application Notes
Plant Tissue Culture Tested
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR’s or other supplements.
The addition of MES will help to stabilize pH fluctuations during media preparation and culture
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.