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Introduction:
Contains the macro- and micronutrients as described by Litvay et al. (1981).
Litvay et al. (1981) originally developed the medium to culture both juvenile and mature tissues of Douglas-fir (Pseudotsuga menziesii) and Loblolly pine (Pinus taeda). The original formulation contained 30 g/L sucrose and the following vitamins (mg/L): 100 myo-Inositol, 0.5 Nicotinic Acid, 0.1 Pyridoxine-HCl, and 0.1 Thiamine-HCl.
L546 Litvay Basal Salt Mixture
Synonym: LM Basal Salt Mixture
| Form: | Fine Powder |
| Appearance: | Off-white to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 4.95g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Litvay et al. (1981). |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 31 |
| Calcium Chloride, Anhydrous | 16.61 |
| Cobalt Chloride.6H2O | 0.125 |
| Cupric Sulfate.5H2O | 0.5 |
| Ferric Sodium EDTA | 36.7 |
| Magnesium Sulfate, Anhydrous | 903.38 |
| Manganese Sulfate.H2O | 21 |
| Molybdic Acid (Sodium Salt). 2H2O | 1.25 |
| Potassium Iodide | 4.15 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 340 |
| Zinc Sulfate.7H2O | 43 |
Application Notes
Plant species: Douglas-fir, Loblolly Pine, Carrot Litvay et al.
(1981) originally developed the medium to culture both juvenile and mature tissues of Douglas-fir (Pseudotsuga menziesii) and Loblolly pine (Pinus taeda). The original formulation contained 30 g/L sucrose and the following vitamins (mg/L): 100 myo-Inositol, 0.5 Nicotinic Acid, 0.1 Pyridoxine•HCl and 0.1 Thiamine•HCl.
This medium was later used to study embryogenesis in wild carrot (Daucus carota L.) suspension cells (Litvay et al, 1985).
References
Litvay, JD, MA Johnson, DC Verma, D Einspahr, K Weyrauch. 1981. Conifer suspension culture medium development using analytical data from developing seeds. Inst.
Paper Chemistry, IPC Tech Paper Ser No 115. Appleton, WI. Litvay, JD, DC Verma, MA Johnson. 1985. Influence of loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.). Plant Cell Rep. 4:325.