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Introduction:
Contains the macro- and micronutrients as described by Murashige and Skoog (1962).
IITA formulation as described by Vuylsteke (1989) (International Institute for Tropical Agriculture).
M462 Musa (Banana) Multiplication Medium IITA Formulation*
Properties
| Form: | Powder |
| Appearance: | White to Off-White |
| Application: | Plant Tissue Culture |
| Solubility: | Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water. |
| Typical Working Concentration: | 36.36 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Formulation as described by Vuylsteke (1989). pH = 3.75 - 4.75 *International Institute for Tropical Agriculture |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2EDTA.2H2O | 37.26 |
| Ferrous Sulfate | 27.85 |
| Magnesium Sulfate, Anhydrous | 180.74 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt).2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| L-Ascorbic Acid | 20 |
| 6-Benzylaminopurine (BA) | 4.5 |
| Gellan Gum - Biotech Grade | 2000 |
| Glycine | 2 |
| Indole-3-acetic Acid | 0.175 |
| Nicotinic Acid (Free Acid) | 0.5 |
| Pyridoxine.HCl | 0.5 |
| Sucrose | 30,000 |
| Thiamine.HCl | 0.4 |
Application Notes
Plant Tissue Culture Tested
References
Vuylsteke D (1989) Shoot-tip culture for the propagation, conservation, and exchange of Musa germplasm. Practical manuals for handling crop germplasm in vitro. 2. Rome:
International Board for Plant Genetic Resources.