Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins.
Contains 20 g/L Sucrose. Also contains: 150 mg/L Citric Acid, 0.3 mg/L IAA, 1.75 mg/L IBA, 1.75 mg/L NAA, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.
Form: | Powder |
Appearance: | White to Off-white Powder |
Application: | Plant Tissue Culture |
Solubility: | Water |
Typical Working Concentration: | 24.57 g/L |
Storage Temp: | 2-8° C |
Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: | Contains the macro- and
micronutrients as described by Murashige and
Skoog (1962). pH = 2.75 - 3.75 |
Formula (mg/L) | |
---|---|
Ammonium Nitrate | 1650 |
Boric Acid | 6.2 |
Calcium Chloride, Anhydrous | 333 |
Cobalt Chloride.6H2O | 0.025 |
Cupric Sulfate.5H2O | 0.025 |
Ferric Sodium EDTA | 36.7 |
Magnesium Sulfate, Anhydrous | 181 |
Manganese Sulfate.H2O | 16.9 |
Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
Potassium Iodide | 0.83 |
Potassium Nitrate | 1900 |
Potassium Phosphate, Monobasic | 170 |
Zinc Sulfate.7H2O | 8.6 |
Citric Acid (Free Acid) Anhydrous | 150 |
Glycine (Free Base) | 2 |
Indole-3-acetic Acid | 0.3 |
Indole-3-butyric Acid | 1.75 |
myo-Inositol | 100 |
a-Naphthaleneacetic Acid | 1.75 |
Nicotinic Acid (Free Acid) | 0.5 |
Pyridoxine.HCl | 0.5 |
Sucrose | 20,000 |
Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
Plant species: Cattleya and other epiphytic orchids.
References
Murashige, T and F Skoog. 1962. A revised
medium for rapid growth and bioassays with
tobacco tissue cultures. Physiol. Plant. 15: 473-497.
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |