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Introduction:
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins.
Contains 20 g/L Sucrose. Also contains: 150 mg/L Citric Acid, 0.3 mg/L IAA, 1.75 mg/L IBA, 1.75 mg/L NAA, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.
M507 Murashige Cattleya Orchid Multiplication Medium
Properties
| Form: | Powder |
| Appearance: | White to Off-white Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 24.57 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and
micronutrients as described by Murashige and
Skoog (1962). pH = 2.75 - 3.75 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 333 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Ferric Sodium EDTA | 36.7 |
| Magnesium Sulfate, Anhydrous | 181 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| Citric Acid (Free Acid) Anhydrous | 150 |
| Glycine (Free Base) | 2 |
| Indole-3-acetic Acid | 0.3 |
| Indole-3-butyric Acid | 1.75 |
| myo-Inositol | 100 |
| a-Naphthaleneacetic Acid | 1.75 |
| Nicotinic Acid (Free Acid) | 0.5 |
| Pyridoxine.HCl | 0.5 |
| Sucrose | 20,000 |
| Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
Plant species: Cattleya and other epiphytic orchids.
References
Murashige, T and F Skoog. 1962. A revised
medium for rapid growth and bioassays with
tobacco tissue cultures. Physiol. Plant. 15: 473-497.