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Introduction:
This medium may be sold as Murashige & Skoog (MS) Shoot Multiplication Medium A by some media suppliers.
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains (mg/L): 170 Sodium Phosphate monobasic, 80 Adenine Hemisulfate, 30.0 2iP, 0.3 IAA, and FeNa-EDTA in place of Ferrous Sulfate and Na2-EDTA.
M527 Murashige Modified Multiplication Basal Medium
| Synonym: | Murashige & Skoog (MS) Shoot Multiplication Medium A |
Properties
| Form: | Fine to Fluffy Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 4.68 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains (mg/L): 170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 30.0 2iP, 0.3 IAA, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA. |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 333 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Ferric Sodium EDTA | 36.7 |
| Magnesium Sulfate, Anhydrous | 181 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt). 2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Sodium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| Adenine Hemisulfate | 80 |
| 6-?,?-Dimethylallylaminopurine (2iP) | 30 |
| Indoleacetic Acid | 0.3 |
| myo-Inositol | 100 |
| Thiamine.HCl | 0.4 |
Application Notes
Plant Tissue Culture Tested
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with
tobacco tissue cultures. Physiol. Plant. 15: 473-497.