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Introduction:
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: No Ammonium Nitrate (A300) or Potassium Nitrate (P100).
M531 Murashige & Skoog (MS) Modified Basal Salt Mixture (No Nitrogen)
Synonym: Modified MS Medium
Properties
| Form: | Fine to Powder |
| Appearance: | White to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 0.78 g/L |
| Storage Temp: | 2-8 °C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: No Ammonium Nitrate or Potassium Nitrate. |
| Formula (mg/L) | |
|---|---|
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2EDTA.2H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt).2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
Application Notes
Plant Tissue Culture Tested
Nitrogen must be supplemented to avoid nitrogen deficiency.
References
Murashige, T and F Skoog. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.