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Introduction:
Contains the macro- and micronutrients and organic constituents as described by Malmgren (1996). Without sucrose and agar.
M534 Malmgren Modified Terrestrial Orchid Medium Without Sucrose and Agar
Properties
| Form: | Powder |
| Appearance: | Gray to Black |
| Application: | Orchid Cultur |
| Solubility: | Water |
| Typical Working Concentration: | 21.84 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro - and micronutrients and organic constituents as described by Malmgren (1996) . Does not contain Sucrose or Agar. |
| Formula (mg/L) | |
|---|---|
| Calcium Phosphate, Tribasic | 75 |
| Na2 EDTA.2H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 97.69 |
| Manganese Sulfate.H2O | 1.54 |
| Potassium Phosphate, Monobasic | 75 |
| Activated Charcoal | 1000 |
| D-Biotin | 0.05 |
| Casein, Enzymatic Hydrolysate | 400 |
| Folic Acid | 0.5 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 5 |
| Pineapple Powder | 20,000 |
| Pyridoxine.HCl | 5 |
| Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
Plant species: Terrestrial orchids including Paphiopedilum, Cypripedium, and Phragmipedium
This medium is a good seed sowing medium for many terrestrial orchid species. Originally
developed for Cypripedium, this medium is also used for germination of Phragmipedium and
Paphiopedilum. Germination may be enhanced by includi
ng 50 ml of Coconut Water (C195) per
liter of medium.
References
Malmgren, S. 1996. In: C. Allen, Editor, North American Native Terrestrial Orchids.
Propagation and Production Conf. Proc., pp 63
-
71.