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Introduction:
Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962) with the following exception: Without Potassium Phosphate Monobasic. Also contains (mg/mL): 300 Sodium Phosphate Monobasic, 150 Adenine Hemisulfate, 1000 Casein, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.
M541 Murashige & Skoog (MS)Modified Basal Medium
Properties
| Form: | Powder |
| Appearance: | White to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 5.69 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962) with the following exceptions: Potassium Phosphate Monobasic is omitted, Sodium Phosphate Monobasic is added, and Ferrous Sulfate and Disodium EDTA are replaced with Ferric Sodium EDTA. Approx. pH = 4.75 ± 0.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride•6H2O | 0.025 |
| Cupric Sulfate•5H2O | 0.025 |
| Ferric Sodium EDTA | 36.7 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate•H2O | 16.9 |
| Molybdic Acid (Sodium Salt)•2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Sodium Phosphate Monobasic | 300 |
| Zinc Sulfate•7H2O | 8.6 |
| Adenine Hemisulfate | 150 |
| Casein, Enzymatic Hydrolysate | 1000 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 5 |
| Pyridoxine•HCl | 1 |
| Thiamine•HCl | 0.5 |
Application Notes
Plant Tissue Culture Tested
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497