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Introduction:
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins. Also contains (mg/L): 2.0 2,4-D, 0.05 Kinetin, and sucrose.
M550 Murashige & Skoog (MS)Modified Medium Arabidopsis Culture Medium
Properties
| Form: | Fine to Fluffy Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 24.44g/L |
| Storage Temp: | 2-8° |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins. Also contains (mg/L): 2.0 2,4-D, 0.05 Kinetin, and sucrose |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2EDTA.2H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt). 2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| 2,4-Dichlorophenoxyacetic Acid | 2.0 |
| Kinetin | 0.05 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 1.0 |
| Pyridoxine•HCl | 1.0 |
| Sucrose | 20,000 |
| Thiamine .HCl | 0.4 |
Application Notes
Plant Tissue Culture Tested
Plant species: Arabidopsis thaliana
This medium was developed for the culture of Arabidopsis callus and cell suspension cultures
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497