Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
Sterile filtered liquid solution containing the Macro- and Micronutrients and Vitamins as described by Murashige & Skoog (1962), then modified to contain 15 g/L sucrose and 15 g/L glucose.
Synonyms: | MS Medium |
Form: | Liquid |
Appearance: | Clear, Colorless to Light Yellow |
Application: | Plant Tissue Culture |
Solubility: | Miscible with Water |
Typical Working Concentration: | 1x |
Storage Temp: | 2-8°C |
Other Notes: | Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). pH = 3.5-4.5 |
Formula (mg/L) | |
---|---|
Ammonium Nitrate | 1650 |
Boric Acid | 6.2 |
Calcium Chloride, Anhydrous | 332.2 |
Cobalt Chloride.6H2O | 0.025 |
Cupric Sulfate.5H2O | 0.025 |
Na2EDTA.2H2O | 37.26 |
Ferrous Sulfate.7H2O | 27.8 |
Magnesium Sulfate, Anhydrous | 180.7 |
Manganese Sulfate.H2O | 16.9 |
Molybdic Acid (Sodium Salt). 2H2O | 0.25 |
Potassium Iodide | 0.83 |
Potassium Nitrate | 1900 |
Potassium Phosphate, Monobasic | 170 |
Zinc Sulfate.7H2O | 8.6 |
Glycine (Free Base) | 2 |
myo-Inositol | 100 |
Nicotinic Acid (Free Acid) | 0.5 |
Pyridoxine.HCl | 0.5 |
Thiamine.HCl | 0.1 |
Sucrose | 30,000 |
Application Notes
Plant Tissue Culture Tested
Plant species: This medium is used for a numerous species.
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with
tobacco tissue cultures. Physiol. Plant. 15: 473-497.
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |