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Introduction:
This medium may be sold as Murashige and Skoog Shoot Multiplication Medium C by some media suppliers. Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains (mg/L): 148 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 1.0 Kinetin, 0.1 NAA, and 30,000 Sucrose.
M555 Murashige & Skoog Modified Multiplication Medium
Properties
| Form: | Fine to Fluffy Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 34.66g/L |
| Storage Temp: | 2-8° |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains (mg/L): 148 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 1.0 Kinetin, 0.1 NAA, and 30,000 Sucrose. |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride•6H2O | 0.025 |
| Cupric Sulfate•5H2O | 0.025 |
| Na2EDTA•2H2O | 37.26 |
| Ferrous Sulfate•7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate•H2O | 16.9 |
| Molybdic Acid (Sodium Salt)• 2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Sodium Phosphate, Monobasic | 148 |
| Zinc Sulfate.7H2O | 8.6 |
| Adenine Hemisulfate | 80 |
| Kinetin | 1.0 |
| myo-Inositol | 100 |
| NAA | 0.1 |
| Sucrose | 30,000 |
| Thiamine.HCl | 0.4 |
Application Notes
Plant Tissue Culture Tested
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.