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Introduction:
Contains the Macro- and Micronutrients and Vitamins as described by Murashige & Skoog (1962). Modified to contain 30 g/L D-Sucrose and 2 g/L Gelzan.
M5825 Murashige & Skoog (MS) Basal Medium w/Sucrose & Gelzan
Synonym: MS Medium w/ Vitamin, Sucrose & Gelzan
Properties
| Form: | Fine Powder |
| Appearance: | Tan Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Partially Soluble Water |
| Typical Working Concentration: | 34.43 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). Approximate unadjusted pH = 3.5-5.0 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2EDTA.2H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate , Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt). 2H2O | 0.25 |
| Gelzan | 2000.0 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| Glycine(Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 0.5 |
| Pyridoxine .HCl | 0.5 |
| Sucrose | 30,000 |
| Thiamine•HCl | 0.1 |
Application Notes
Plant Tissue Culture Tested
Plant species: This medium is used for a numerous species.
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.