Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
Contains the macro- and micronutrients, and organic constituents as described by Lichter (1982). Without sucrose.
This medium was derived from that described by Nitsch & Nitsch and was developed to support the initiation and growth of haploid plants from anther and pollen cultures of Brassica napus. This medium contains the equivalent of 500 mg/L Calcium Nitrate tetrahydrate as described in the original formulation.
Form: | Powder |
Appearance: | White to Yellow Powder |
Application: | Plant Tissue Culture |
Solubility: | Water |
Typical Working Concentration: | 1.77g/L |
Storage Temp: | 2-8°C |
Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: | Contains the macro- and micronutrients and organic constituents as described by Lichter (1982). |
Formula (mg/L) | |
---|---|
Boric Acid | 10 |
Calcium Nitrate (dried) | 347 |
Cobalt Chloride.6H2O | 0.025 |
Cupric Sulfate.5H2O | 0.025 |
Ferric Sodium EDTA | 36.7 |
Magnesium Sulfate, Anhydrous | 61 |
Manganese Sulfate.H2O | 18.95 |
Molybdic Acid (Sodium Salt).2H2O | 0.25 |
Potassium Nitrate | 125 |
Potassium Phosphate, Monobasic | 125 |
Zinc Sulfate.7H2O | 10 |
D-Biotin | 0.05 |
Folic Acid | 0.5 |
L-Glutamine | 800 |
Glutathione | 30 |
Glycine (Free Base) | 2 |
myo-Inositol | 100 |
Nicotinic Acid (Free Acid) | 5 |
Pyridoxine.HCl | 0.5 |
L-Serine | 100 |
Thiamine.HCl | 0.5 |
Application Notes
Plant Tissue Culture Tested Plant species: Brassica napus This medium was derived from that described by Nitsch & Nitsch and was developed to support the initiation and growth of haploid plants from anther and pollen cultures of Brassica napus. This medium contains the equivalent of 500 mg/L calcium nitrate tetrahydrate as described in the original formulation.
References
Lichter, R. 1981. Z. Pflanzenphysiol. 103: 119. Lichter, R. 1982. Z. Pflanzenphysiol. 105: 427
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |