Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
Modified Chu/Gamborg basal medium. Contains the macronutrients described by Chu (1975) & the micronutrients described by Gamborg, et al. (1968).
Form: | Powder |
Appearance: | White to Yellow Powder |
Application: | Plant Tissue Culture |
Solubility: | Water |
Typical Working Concentration: | 4.10g/L |
Storage Temp: | 2-8°C |
Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: | Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5 |
Formula (mg/L) | |
---|---|
Ammonium Sulfate | 463 |
Boric Acid | 3 |
Calcium Chloride, Anhydrous | 125.33 |
Cobalt Chloride.6H2O | 0.025 |
Cupric Sulfate.5H2O | 0.025 |
Na2 EDTA.2H2O | 37.26 |
Ferrous Sulfate.7H2O | 27.8 |
Magnesium Sulfate, Anhydrous | 90.37 |
Manganese Sulfate.H2O | 10 |
Molybdic Acid (Sodium Salt).2H2O | 0.25 |
Potassium Iodide | 0.75 |
Potassium Nitrate | 2830 |
Potassium Phosphate, Monobasic | 400 |
Zinc Sulfate.7H2O | 2 |
myo-Inositol | 100 |
Nicotinic Acid (Free Acid) | 1 |
Pyridoxine.HCl | 1 |
Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659- 668.
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |