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Introduction:
Contains the vitamins as described by Nitsch and Nitsch (1969).
myo-Inositol may precipitate out of solution when cold; warming with occasional agitation will redissolved the precipitate. Use at a concentration of 1 mL/L of medium to achieve the proper final concentration.
N603 Nitsch & Nitsch Vitamin Solution (1000x)
Properties
| Form: | Liquid |
| Appearance: | Clear, Colorless Solution |
| Application: | Plant Tissue Culture |
| Solubility: | Miscible with Water |
| Typical Working Concentration: | 1 mL/ L of Medium |
| Storage Temp: | 2 - 8° C |
| Storage Temp of Stock Solution: | 2-8° C |
| Other Notes: | Contains the vitamins as described by Nitsch and Nitsch (1969). Myo-Inositol may precipitate out of solution when cold; warming with occasional agitation will redissolved the precipitate. |
| Formula (mg/L) | |
|---|---|
| D-Biotin | 50 |
| Folic Acid | 500 |
| Glycine (Free Base) | 2000 |
| myo-Inositol | 100,000 |
| Nicotinic Acid (Free Acid) | 5000 |
| Pyridoxine.HCl | 500 |
| Thiamine.HCl | 500 |
Application Notes
Plant Tissue Culture Tested
References
Nitsch, JP and C Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87.