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Introduction:
Contains the vitamins as described by Nitsch and Nitsch (1969).
Instructions for dissolving the powder: Add 5 mL 1N KOH or NaOH per 100 mL package or approx 13 mL per 250 mL package. Stir until suspension dissolves; add additional base if necessary. Bring to volume with water.
This product can be filter sterilized and stored in the refrigerator. Alternatively, it can be stored in aliquots in the freezer for long-term. Freezer storage of non-sterile solutions is preferred as vitamins are subject to breakdown by bacteria and fungi. myo-Inositol may precipitate out of solution when cold; warming with occasional agitation will redissolved the precipitate.
N608 Nitsch & Nitsch Vitamin Powder (1000x)
| Form: | Powder |
| Appearance: | White to Off-white powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 10.86 g/ 100 mL produces a 1000X concentrate; 1 mL/ L of medium when dissolved in 1N KOH and water to the volume indicated on the package. See instructions below. |
| Storage Temp: | 2 - 8 °C |
| Storage Temp of Stock Solution: | 2 - 8 °C |
| Other Notes: | Contains the vitamins as described by Nitsch and Nitsch (1969). Instructions for dissolving the powder: Add 5 mL 1N KOH or NaOH per 100 mL package or approx 13 mL per 250 mL package. Stir till suspension dissolves; add additional base if necessary. Bring to volume with water. This product can be filter sterilized and stored in the refrigerator. Alternatively, it can be stored in aliquots in the freezer for long-term. Freezer storage of non-sterile solutions is preferred as vitamins are subject to breakdown by bacteria and fungi. Myo-Inositol may precipitate out of solution when cold; warming with occasional agitation will redissolved the precipitate. |
| Formula (mg/L) | |
|---|---|
| D-Biotin | 50 |
| Folic Acid | 500 |
| Glycine (Free Base) | 2000 |
| myo-Inositol | 100,000 |
| Nicotinic Acid (Free Acid) | 5000 |
| Pyridoxine.HCl | 500 |
| Thiamine.HCl | 500 |
Application Notes
Plant Tissue Culture Tested
References
Nitsch, JP and C Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87.