Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
Contains the macro- and micronutrients as described by Nitsch and Nitsch (1969).
This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
Form: | Powder |
Appearance: | White to Yellow Powder |
Application: | Plant Tissue Culture |
Solubility: | Water |
Typical Working Concentration: | 2.10g/L |
Storage Temp: | 2-8°C |
Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: | Contains the macro- and micronutrients as described by Nitsch and Nitsch (1969) pH = 3.5 – 4.5 |
Formula (mg/L) | |
---|---|
Ammonium Nitrate | 720 |
Boric Acid | 10 |
Calcium Chloride, Anhydrous | 166 |
Cupric Sulfate.5H2O | 0.025 |
Na2 EDTA.2H2O | 37.26 |
Ferrous Sulfate.7H2O | 27.8 |
Magnesium Sulfate, Anhydrous | 90.372 |
Manganese Sulfate.H2O | 18.9 |
Molybdic Acid (Sodium Salt).2H2O | 0.25 |
Potassium Nitrate | 950 |
Potassium Phosphate, Monobasic | 68 |
Zinc Sulfate.7H2O | 10 |
Application Notes
Plant Tissue Culture Tested Plant species: Nicotiana (tobacco) This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
References
Nitsch, JP and C Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |