Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |
Contains the macro- and micronutrients, and vitamins as described by Nitsch and Nitsch (1969).
This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
Properties | |
---|---|
Form: | Powder |
Appearance: | White to Yellow Powder |
Application: | Plant Tissue Culture |
Solubility: | Water |
Typical Working Concentration: | 2.21g/L |
Storage Temp: | 2-8°C |
Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: | Contains the macro- and micronutrients and vitamins as described by Nitsch and Nitsch (1969) |
Formula (mg/L) | |
---|---|
Ammonium Nitrate | 720 |
Boric Acid | 10 |
Calcium Chloride, Anhydrous | 166 |
Cupric Sulfate.5H2O | 0.025 |
Na2 EDTA.2H2O | 37.26 |
Ferrous Sulfate.7H2O | 27.8 |
Magnesium Sulfate, Anhydrous | 90.372 |
Manganese Sulfate.H2O | 18.9 |
Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
Potassium Nitrate | 950 |
Potassium Phosphate, Monobasic | 68 |
Zinc Sulfate.7H2O | 10 |
D-Biotin | 0.05 |
Folic Acid | 0.5 |
Glycine (Free Base) | 2 |
myo-Inositol | 100 |
Nicotinic Acid (Free Acid) | 5 |
Pyridoxine.HCl | 0.5 |
Thiamine.HCl | 0.5 |
Application Notes
Plant Tissue Culture Tested
Plant species: Nicotiana (tobacco) This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
References
Nitsch, JP and C Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87.
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# [email protected] |