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Introduction:
This medium is a modification of our original orchid replate medium (P668) except without charcoal (and agar). This medium permits the culture of orchids in a liquid system or for orchid species that do not require charcoal to neutralize phenolics produced by the plants.
O139 Orchid Maintenance/Replate Medium Without Charcoal and Agar
Properties
| Form: | Powder |
| Appearance: | White to Yellow powder |
| Application: | Orchid Culture |
| Solubility: | Water |
| Typical Working Concentration: | 25.31 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | pH = 4.75 - 5.7 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 825 |
| Boric Acid | 3.1 |
| Calcium Chloride, Anhydrous | 166 |
| Cobalt Chloride.6H2O | 0.0125 |
| Cupric Sulfate.5H2O | 0.0125 |
| Na2EDTA.2H2O | 37.3 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 90.35 |
| Manganese Sulfate.H2O | 8.45 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.125 |
| Potassium Iodide | 0.415 |
| Potassium Nitrate | 950 |
| Potassium Phosphate, Monobasic | 85 |
| Zinc Sulfate.7H2O | 5.3 |
| MES (Free Acid) | 1000 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 1 |
| Peptone from Meat | 2000 |
| Pyridoxine.HCl | 1 |
| Sucrose | 20,000 |
| Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
Plant species: Many epiphytic orchid species
This medium is a modification of our original orchid replate medium
(P668) except without charcoal (and agar). This medium permits the culture of orchids in a liquid system or for orchid species that do not require charcoal to
neutralize phenolics produced by the plants.