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Introduction:
This medium is a modification of our Orchid Multiplication Medium (P723) by the deletion of charcoal. This medium should work well for the propagation of Phalaenopsis and Dendrobium stem props.
O753 Orchid Multiplication Medium Contains Agar, Without Charcoal Mother Flasking Medium III
Properties
| Form: | Powder |
| Appearance: | White to YellowPowder |
| Application: | Orchid Cultur |
| Solubility: | Hot Water |
| Typical Working Concentration: | 32.30 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | pH = 4.75 – 5.75 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 825 |
| Boric Acid | 3.1 |
| Calcium Chloride, Anhydrous | 166 |
| Cobalt Chloride.6H2O | 0.0125 |
| Cupric Sulfate.5H2O | 0.0125 |
| Na2EDTA.2H2O | 37.3 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate,Anhydrous | 90.35 |
| Manganese Sulfate.H2O | 8.45 |
| Molybdic Acid, Sodium Salt.2H2O | 0.125 |
| Potassium Iodide | 0.415 |
| Potassium Nitrate | 950 |
| Potassium Phosphate, Monobasic | 85 |
| Zinc Sulfate.7H2O | 5.3 |
| Agar | 7000 |
| 6-Benzylaminopurine (BA) | 2 |
| MES (Free Acid) | 1000 |
| myo-Inositol | 100 |
| a-Naphthaleneacetic Acid | 0.5 |
| Nicotinic Acid (Free Acid) | 0.5 |
| Peptone from Meat | 2000 |
| Pyridoxine.HCl | 0.5 |
| Sucrose | 20,000 |
| Thiamine.HCl | 1 |
Application Notes
Plant Tissue Culture Tested
Plant species: Many epiphytic orchid species
This medium is a modification of our Orchid Multiplication Medium (P72
3) by the deletion of
charcoal. This medium should work well for the propagation of Phal
aenopsis and Dendrobium
stem props.